Welcome to the forums at seaphages.org. Please feel free to ask any questions related to the SEA-PHAGES program. Any logged-in user may post new topics and reply to existing topics. If you'd like to see a new forum created, please contact us using our form or email us at info@seaphages.org.
Recent Activity
Viknesh Sivanathan posted in did you know you can do restriction digests in the microwave?
nic.vega posted in did you know you can do restriction digests in the microwave?
nic.vega posted in did you know you can do restriction digests in the microwave?
Viknesh Sivanathan posted in did you know you can do restriction digests in the microwave?
nic.vega posted in did you know you can do restriction digests in the microwave?
Does GeneMark identify all potential start sites?
| Link to this post | posted 21 Jun, 2018 20:18 | |
|---|---|
|
In looking at the coding potential for potential start sites, I was under the assumption that GeneMark identified all potential start sites (not the coding potential, but the ATG, TTG and GTG start sites). For this gene, the start with the longer ORF at 41175, which is annotated more often, does not show up on the GeneMark output. Is there a reason GeneMark would omit a potential start site? Maybe it does this and I haven't noticed in the past. |
46Kb
39Kb| Link to this post | posted 21 Jun, 2018 21:56 | |
|---|---|
|
Evan, GeneMark does call all starts ATG, GTG, and TTG (you will find that GenMark can agree with Glimmer on a TTG start in DNA Master). However, GeneMrk's graphic output is only coded to represent ATG (longer upticks) and TTG (shorter upticks) starts. I think they ran out of money to update all of the graphic displays….. |
| Link to this post | posted 22 Jun, 2018 11:08 | |
|---|---|
|
|
That makes sense, thanks Debbie! |