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uOttawaPHAGE posted in Fin anti-sigmaF factor
Debbie Jacobs-Sera posted in Weird domain distribution over "cytosine methyltransferase" hits in an AS3 phage
nic.vega posted in Weird domain distribution over "cytosine methyltransferase" hits in an AS3 phage
Debbie Jacobs-Sera posted in Weird domain distribution over "cytosine methyltransferase" hits in an AS3 phage
nic.vega posted in Weird domain distribution over "cytosine methyltransferase" hits in an AS3 phage
Replication initiator protein A
| Link to this post | posted 02 Jun, 2018 17:36 | |
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I have seen good HHpred matches to this protein in a number of phages. In Gordonia phage TimTam and Eviarto, I see a nice match with 97.2% probability and 36% coverage. Here's the definition This entry represents the N-terminal domain of a small family of phage proteins. The protein contains a region of low-complexity sequence that reflects DNA direct repeats able to function as an origin of phage replication. The region is N-terminal to the low-complexity region. Can call eitehr N-terminal phage replisome organiser or Replication initiator protein A But can also go with more generic HTH DNA Binding domain protein |
| Link to this post | posted 02 Jun, 2018 17:38 | |
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PS In both Gordonia phages Eviarto and TimTam, this gene is followed by the replisome organizer (helicase loader/inhibitor protein) so that function should also be added to the list |
| Link to this post | posted 04 Jun, 2018 12:52 | |
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Is this the same as RepA? I think we have that already. IF not, how is it different? |
| Link to this post | posted 04 Jun, 2018 17:42 | |
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So the HHPred match with a link to a pdb file does give me repA https://www.rcsb.org/structure/4PT7 the short gene that follows also has nice matches to Bacillus subtilis replisome organizer so this protein may be coded by two genes but I will just call function for the first gene! |