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Blast error

| posted 04 Sep, 2020 13:28
Hi all,
Sorry about the timing.
In talking with Dr. Lawrence, we think this is an NCBI change. I have written emails to any/all folks I can think of at NCBI who may be able to answer or direct me to who could answer the question. NLM Help Desk replied with a 2-5 response timeline. I'll keep you posted.
debbie
| posted 04 Sep, 2020 14:12
Thanks, Debbie.

Sorry to add to your plate. Never rains, but it pours.

Steve
| posted 04 Sep, 2020 14:15
No worries. Par for the course.
| posted 05 Sep, 2020 13:16
An update to DNA Master has been posted. Blast will not work unless you update.
The latest version is Version 5.23.3 Build 2657 4Sep2020
| posted 05 Sep, 2020 23:24
Thanks Debbie! I have the updated version but I'm still getting the error with SSL when I try to blast just one gene.
| posted 05 Sep, 2020 23:38
Oh never mind! It's working now smile
| posted yesterday, 13:01
I am getting the error quoted at the bottom of this from Steve Caruso. I tried a couple of other fixes that are in the forum regarding SSL/DNA BLAST errors: the Windows library and the Bitvise SSH client. The prose regarding the Windows library fix is copied below. Since neither of these things work, any ideas?
DanRussell
Hi Katie,

I think this error is due to not having a certain library installed on the particular version of Windows that's running. That library wasn't required until after the update earlier this year to use secure NCBI servers. I think I came across that error myself, and in my notes I have that I went to the link below and installed the Visual C++ package there, then restarted and it worked.

https://www.microsoft.com/en-us/download/details.aspx?id=5555

UPDATE Sep 2021: The link above no longer works, but the file can be found at the link below now. Download it from within Windows and then run it, and make sure you close DNA Master and restart Windows before trying to BLAST again.

https://phagesdb.org/static/vcredist_x86.exe

Hopefully that helps,
–Dan

scaruso
All,

I am getting the following error when I try to blast a gene in DNAM on my PC running W10 (not a surface).

Error connecting with SSL.
error: 1409442E:SSL routines::SSL3_READ_BYTES:tlsv1 alert protocol version.

Anyone else having any trouble or aware of anything going on? Or any ideas?

Steve
 
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