Link to this post | posted 04 Sep, 2020 13:28
debbie	Hi all, Sorry about the timing. In talking with Dr. Lawrence, we think this is an NCBI change. I have written emails to any/all folks I can think of at NCBI who may be able to answer or direct me to who could answer the question. NLM Help Desk replied with a 2-5 response timeline. I'll keep you posted. debbie

Link to this post | posted 04 Sep, 2020 14:12
scaruso	Thanks, Debbie. Sorry to add to your plate. Never rains, but it pours. Steve

Link to this post | posted 04 Sep, 2020 14:15
debbie	No worries. Par for the course.

Link to this post | posted 05 Sep, 2020 13:16
debbie	An update to DNA Master has been posted. Blast will not work unless you update. The latest version is Version 5.23.3 Build 2657 4Sep2020

Link to this post | posted 05 Sep, 2020 23:24
JChahal	Thanks Debbie! I have the updated version but I'm still getting the error with SSL when I try to blast just one gene.

Link to this post | posted 05 Sep, 2020 23:38
JChahal	Oh never mind! It's working now

Link to this post | posted 05 Apr, 2025 13:01

rdejong

I am getting the error quoted at the bottom of this from Steve Caruso. I tried a couple of other fixes that are in the forum regarding SSL/DNA BLAST errors: the Windows library and the Bitvise SSH client. The prose regarding the Windows library fix is copied below. Since neither of these things work, any ideas? DanRussell Hi Katie, I think this error is due to not having a certain library installed on the particular version of Windows that's running. That library wasn't required until after the update earlier this year to use secure NCBI servers. I think I came across that error myself, and in my notes I have that I went to the link below and installed the Visual C++ package there, then restarted and it worked. https://www.microsoft.com/en-us/download/details.aspx?id=5555 UPDATE Sep 2021: The link above no longer works, but the file can be found at the link below now. Download it from within Windows and then run it, and make sure you close DNA Master and restart Windows before trying to BLAST again. https://phagesdb.org/static/vcredist_x86.exe Hopefully that helps, –Dan scaruso All, I am getting the following error when I try to blast a gene in DNAM on my PC running W10 (not a surface). Error connecting with SSL. error: 1409442E:SSL routines::SSL3_READ_BYTES:tlsv1 alert protocol version. Anyone else having any trouble or aware of anything going on? Or any ideas? Steve

Link to this post | posted 07 Apr, 2025 16:57
debbie	Randy, Is this so you can submit your genomes for QC? If you can't reBLAST on DNA Master, just record that on your annotation sheet and submit. best, debbie

Link to this post | posted 07 Apr, 2025 17:14
rdejong	Thanks, yes, that is the purpose. I considered that option, but found that I was able to avoid this error by doing an install on a different computer. It happens to be Windows 10 instead of Windows 11, for what it's worth. Genome submitted with reBLAST complete!

Link to this post | posted 07 Apr, 2025 18:23
debbie	Thanks so much! Gold star for getting it re-blasted! best, debbie