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Recent Activity
Tail Assembly Chaperone- Translational Frameshift
| Link to this post | posted 03 Jun, 2023 16:11 | |
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All AS phages found so far have a tail assembly chaperone preceding the tape measure protein with a programmed translational frameshift. The tail assembly chaperone is typically found in the left arm of the genome around gp13-15. It is a -1 frameshift: G_GG.G_GA.A |
| Link to this post | posted 19 Feb, 2025 18:00 | |
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I think I have found a frameshift in HamCheese tail assembly chaperone protein. I watched the helpful video for inputting a frameshift in DNA master but am still getting an error message. Gene 13 5'- 9060 to 3'- 9413 Gene 14 5'-9386 to 3'- 9784 The GGGAA runs from 5'- 9383 to 3'-9387 In the regions table for gene 14 I added the table (for which the length is divisible by 3) Start: 9060 Stop: 9383 Start: 9383 Stop: 9784 However, when I press validate I get an incorrect region count. Can anyone help me find the error I am missing? |
312Kb| Link to this post | posted 19 Feb, 2025 18:37 | |
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Can you show what's on the "Regions" tab for the gene? I'm guessing that the lengths for each region are possibly not correct there. Lee |
| Link to this post | posted 19 Feb, 2025 19:00 | |
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Of course! Regions screen shot attached |
228Kb| Link to this post | posted yesterday, 15:52 | |
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Hmmm - I don't see anything wrong there. The screenshot doesn't show the bottom of the page, but does it show the two regions as bars? As well, does your description page for the gene have the correct start/stop for the longer product, as well as "2" in the regions box? It seems odd that the "incorrect" notice doesn't show any coordinates, just zeroes. I wish I was able to be more helpful! Lee |
| Link to this post | posted yesterday, 21:29 | |
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Hi all, Here are my 2 cents. See attached. debbie |
145Kb