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Other M. smegmatis strains

Link to this post \| posted 23 Oct, 2016 00:20
JBechelli	Our school is pilotting the program this year before officially applying to join the SEA-PHAGES. The M. smegmatis that we currently have is NCTC 8159 [Cornell 3] or 19420 from ATCC. I know the program uses M. smegmatis mc2155 for most of the hosts. Does anyone have any experience using other strains? Our budget is limited and if I don't have to purchase the mc2155 strain it would help us out significantly. Thanks, Jeremy

Link to this post | posted 23 Oct, 2016 00:20

JBechelli

Our school is pilotting the program this year before officially applying to join the SEA-PHAGES. The M. smegmatis that we currently have is NCTC 8159 [Cornell 3] or 19420 from ATCC. I know the program uses M. smegmatis mc2155 for most of the hosts. Does anyone have any experience using other strains? Our budget is limited and if I don't have to purchase the mc2155 strain it would help us out significantly.
Thanks,
Jeremy

Link to this post \| posted 25 Oct, 2016 20:07
debbie	Jeremy, If you join the program (and go through our training) we set up an MTA between U of Pittsburgh and your school to be able to ship M. smegmatis mc2 155 to you. So you could use your bacteria to pilot and then you will have smeg by August of next year (assuming you are accepted into the program).

Link to this post \| posted 26 Oct, 2016 03:16
JBechelli	Thank you Debbie, I think we will try using our current strain. Jeremy