SEA-PHAGES | All posts created by sklambet@ncsu.edu

Link to this post \| posted 03 Apr, 2025 12:53
sklambet@ncsu.edu	Thank you for this option! I have downloaded DNAMaster using Whisky on my mac (with Patrick's helpful guide). Unfortunately when I try to update DNA master I get an ftp error preventing me from setting the preferences correctly. I found instructions for this error for windows users but I am not sure how to navigate that using Whisky. Can anyone help? plyp Hello all, We've had issues with running a full VM with some laptops that are near full or shipped with the lowest storage tiers and can't afford to run an entire virtual machine plus windows just for one software. I found and tested a new app called Whisky that's extremely simple to use and very lightweight. I haven't found any real bugs so far (I get a glimmer error at this moment but the glimmer calls are still being made, it seems to be more related to gene mark and the exact same error is currently happening on real windows machines) The Whisky application can be downloaded from: https://getwhisky.app/ Tested confirmed features: • Preferences are saved between launches • Auto update to 2705 • Gene starts called the same way • BLAST connections work • Fasta/dnam5 files can be easily saved and are accessible directly through Finder for sharing (Open C: drive on startup screen) • Maximum RAM usage at 900MB, Maximum disk usage below 1.4GB Im attaching a quick guide with screenshot instructions Patrick Łypaczewski

Link to this post | posted 03 Apr, 2025 12:53

Thank you for this option! I have downloaded DNAMaster using Whisky on my mac (with Patrick's helpful guide). Unfortunately when I try to update DNA master I get an ftp error preventing me from setting the preferences correctly. I found instructions for this error for windows users but I am not sure how to navigate that using Whisky. Can anyone help?

plyp
Hello all,
We've had issues with running a full VM with some laptops that are near full or shipped with the lowest storage tiers and can't afford to run an entire virtual machine plus windows just for one software. I found and tested a new app called Whisky that's extremely simple to use and very lightweight. I haven't found any real bugs so far (I get a glimmer error at this moment but the glimmer calls are still being made, it seems to be more related to gene mark and the exact same error is currently happening on real windows machines)
The Whisky application can be downloaded from:
https://getwhisky.app/

Tested confirmed features:
• Preferences are saved between launches
• Auto update to 2705
• Gene starts called the same way
• BLAST connections work
• Fasta/dnam5 files can be easily saved and are accessible directly through Finder for sharing (Open C: drive on startup screen)
• Maximum RAM usage at 900MB, Maximum disk usage below 1.4GB
Im attaching a quick guide with screenshot instructions

Patrick Łypaczewski

Posted in: DNA Master → DNA Master on M1 Mac

Link to this post \| posted 05 Mar, 2025 20:31
sklambet@ncsu.edu	Just wanted to share a print file for a phage shaped cookie cutter! See photo for experimental results (cookie) https://www.thingiverse.com/thing:2306864 4.51Mb

Posted in: General Message Board → Phage cookies

Link to this post \| posted 28 Jan, 2025 18:20
sklambet@ncsu.edu	Bacto peptone also solved the precipitation issues last semester for me! Thank you Cole for working on this.

Posted in: Phage Discovery/Isolation → Precipitation of CaCl2 in PYCA top-agar

Link to this post \| posted 17 Oct, 2024 16:54
sklambet@ncsu.edu	sklambet@ncsu.edu Dan, Let me know if you need an email address or other information for data sharing Thanks! You already sent it… thank you so much!

Posted in: Arthrobacter → Genome Sequence Arthrobacter globiformis NRRL B-2979

Link to this post \| posted 17 Oct, 2024 16:53
sklambet@ncsu.edu	Dan, Let me know if you need an email address or other information for data sharing Thanks!

Posted in: Arthrobacter → Genome Sequence Arthrobacter globiformis NRRL B-2979

Link to this post \| posted 26 Sep, 2024 16:36
sklambet@ncsu.edu	Hi Dan, That would be great if you could email the contigs! It would be helpful for nanopore sequecning reads at NCSU that have host contamination Thank you! Stephanie

Posted in: Arthrobacter → Genome Sequence Arthrobacter globiformis NRRL B-2979

Link to this post \| posted 25 Sep, 2024 15:12
sklambet@ncsu.edu	Is the genome sequence of this host strain available? I can't find it on NCBI or other online sources. Thanks!

Posted in: Arthrobacter → Genome Sequence Arthrobacter globiformis NRRL B-2979

Link to this post \| posted 03 Sep, 2024 14:12
sklambet@ncsu.edu	Thank you all so much!!! This is super helpful. Also no apologies needed this response was surprising fast. Stephanie Edited 03 Sep, 2024 14:12

Posted in: Phage Discovery/Isolation → Precipitation of CaCl2 in PYCA top-agar

Link to this post \| posted 03 Sep, 2024 09:34
sklambet@ncsu.edu	I am also having this problem with top agar. I am going to remake this morning and let the media cool more before adding the CaCl2 as suggested. Is there any more information about this since last year? Thanks!

Posted in: Phage Discovery/Isolation → Precipitation of CaCl2 in PYCA top-agar

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