Hi Tammy,

I noticed your inquiry about including sequencing and assembly details in your MRA. DanRussell provided excellent guidance regarding the Illumina sequencing platforms and the associated quality control measures. To further enhance your MRA, consider the following additions:

    Sequencing Platform Details: Specify the exact Illumina platform used (e.g., MiSeq or NextSeq 1000) and the sequencing kit version to ensure clarity. Quality Control Procedures: Detail any quality control steps undertaken, such as adapter trimming, quality filtering, and read length distribution analysis. Assembly Parameters: Mention the assembly parameters used, including the assembler version and any specific settings that were adjusted. Genomic Terminus Determination: Provide a brief explanation of how the genomic termini were determined, referencing any standard protocols or publications if applicable.

    By incorporating these details, your MRA will offer a comprehensive overview of the sequencing and assembly processes, aligning with the expectations outlined by DanRussell.
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