Hi all,
We have some questions on 2 gene starts in PensacolaC28

The first is gene 1 (stop 1611). Glimmer calls the start at 37, and GM at 1. I thought conventionally gene 1 started at nucleotide 1, but RBS favors start at 37. We are wondering if RBS only favors start 2 because it can't accurately calculate where the circular genome is cut?

Next we have Gene 14 (12501 stop). We believe 16 is not a gene, and plan to delete it. On gene 14 glimmer calls the second start. but calling the first start at 12172 eliminates a 100bp gap, however the glimmer start has a great score. It also does not include any additional GM coding potential. We're pretty torn on this call

Any advice is appreciated, thanks!
Emily