Link to this post | posted 16 Mar, 2016 13:01
dedrick	We use a Mini-bead beater from Biospec Products, Inc. I'm not so sure vortexing would do the trick. Bekah Dedrick

Link to this post | posted 16 Mar, 2016 11:39
dedrick	Yes I did. I attached the protocol here. Bekah Dedrick 116Kb

Link to this post | posted 15 Mar, 2016 18:34

dedrick

Hi Dan, Yep, we use the Qiagen RNeasy kit. It's super easy and relatively inexpensive. The kit has you resuspend your cell pellet in a buffer called RLT. We place this suspension in a matrix lysing tube (matrix B - MP Biomedicals 6911-100) and bead beat for 45 seconds on max speed, two times (icing in between) to break open the cells. Then, you centrifuge this tube briefly and transfer the supernatent into a new tube and go on with the procedure where you add 560ul of 80% EtOH. Let me know if you need more information!! Bekah Bekah Dedrick

Link to this post | posted 17 Sep, 2015 00:24
dedrick	Hi Lee, Do you use this method for all Streptomyces species? Are you using liquid media specific for sporulation? I know some do not sporulate in liquid (coelicolor)…not sure if it matters. I have always used spore stocks and top agar. Of course your method sounds easier!!!! Best, Bekah Bekah Dedrick

Link to this post | posted 16 Sep, 2015 14:36
dedrick	Hi Steve, Why aren't you using top agar? I have used it when isolating S. lividans phage. Bekah Bekah Dedrick