Link to this post | posted 12 Aug, 2023 00:24
clfleischacker	Worked well, thanks for the help.

Link to this post | posted 11 Aug, 2023 00:09
clfleischacker	I just installed DNA Master on my new personal computer and the 5.22.5 version was loaded. I tried to update and am receiving the FTP error. Should I contact Kristen? I am just finishing up a genome and can try using a different computer but I am hoping to resolve this issue on my new computer (Windows 11).

Link to this post | posted 17 Jul, 2018 20:38
clfleischacker	I followed the advice from Karen above but the validation now says incorrect length for this gene. I have pressed the calculator button, etc. with no changes. Any other suggestions?

Link to this post | posted 17 Jul, 2018 19:56
clfleischacker	Is the last, wrap-around gene an assumed gene for C1 cluster phages? I deleted it earlier in my annotation but need to know if I should add it back in. Chris

Link to this post | posted 04 Aug, 2017 02:39
clfleischacker	Here is the master file, I am working on the screen shots. 863Kb

Link to this post | posted 04 Aug, 2017 02:36
clfleischacker	For the B1 subcluster phage Lulumae, for gene 67 it has RNaseE as a function which is not on the official function list. Can this be added to the list?

Link to this post | posted 04 Aug, 2017 02:32
clfleischacker	For the B1 subcluster phage Lulumae, for gene 19 it has the queuine tRNA ribosyltransferase as a function which is not on the official function list. Can this be added to the list?

Link to this post | posted 23 Sep, 2016 02:15

clfleischacker

Ok, we are trouble shooting this week since we are not getting plaques. If we do indeed have smeg then we are thinking that our smeg cultures are not dense enough to support plaques. We set up cultures with D29 but did not see any growth of the bacteria and therefore no plaques. hopefully we will know soon if our bacteria is good as we are further growing it up this week to increase its density. Then we will test it with the D29 again. Do people often see contamination with smeg when they grow it on plates with antibiotics? Also, if we see small white pin prick dots on our smeg and phage lysate plates cultured in soft agar, is that the Smeg? As you can see my concerns are mostly about my ability to recognize true growth of smeg. I guess I will just wait to test the more dense smeg cultures with the D29 and see what happens. I will let you know if we have any more problems, hopefully we can solve this one soon! Thanks for your speedy reply! Christine

Link to this post | posted 22 Sep, 2016 16:54

clfleischacker

My original plating of mSmeg from the stock sent to me yielded colonies with a yellowish color and looked dry and had irregular borders. Now, my smeg on the same type of agar plate (taken from the same stock tube) looks white with very small colonies. I am worried that I am growing the wrong type of bacteria! Does msmeg look different over time? These small white colonies did not appear until about 3 days after inoculation. We are also having trouble seeing any plaques: none from direct isolation and none so far from the enriched isolation (2 days incubation with 1 day post- spot test). Any suggestions as to how to proceed? Should I restart the msmeg culture from the yellow cultures or the white cultures? Christine Fleischacker Univ. of Mary