Welcome to the forums at seaphages.org. Please feel free to ask any questions related to the SEA-PHAGES program. Any logged-in user may post new topics and reply to existing topics. If you'd like to see a new forum created, please contact us using our form or email us at info@seaphages.org.

Recent Activity

All posts created by bavina

Link to this post \| posted 20 Sep, 2024 14:07
bavina	Hello, I am also having issues with my PYCa media. I made a batch of broth and top agar and they both became cloudy after adding the CaCl2 solution. There aren't any huge flaky crystals but still a visible change the media almost immediately after adding 1M CaCl2. I waited until the solutions were cooled to 55 C but this still happened. Is this media unusable or can it still be used for experiments? Is the only fix switching to Bacto Peptone like described in the earlier posts? 3.14Mb

Link to this post | posted 20 Sep, 2024 14:07

bavina

Hello,

I am also having issues with my PYCa media. I made a batch of broth and top agar and they both became cloudy after adding the CaCl2 solution. There aren't any huge flaky crystals but still a visible change the media almost immediately after adding 1M CaCl2. I waited until the solutions were cooled to 55 C but this still happened. Is this media unusable or can it still be used for experiments? Is the only fix switching to Bacto Peptone like described in the earlier posts?

Posted in: Phage Discovery/Isolation → Precipitation of CaCl2 in PYCA top-agar