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Recent Activity

All posts created by bavina

Link to this post \| posted 28 Jan, 2025 16:46
bavina	Hello, I wanted to share that switching to Bacto Peptone really helped with out CaCl2 issues! With this brand of peptone, our top agar was more stable and was able to remain in the water bath for around a week. It did eventually crash out of solution over the weekend, but that could have been due to the water level getting low and the bath overheating the agar.

Posted in: Phage Discovery/Isolation → Precipitation of CaCl2 in PYCA top-agar

Link to this post \| posted 20 Sep, 2024 14:07
bavina	Hello, I am also having issues with my PYCa media. I made a batch of broth and top agar and they both became cloudy after adding the CaCl2 solution. There aren't any huge flaky crystals but still a visible change the media almost immediately after adding 1M CaCl2. I waited until the solutions were cooled to 55 C but this still happened. Is this media unusable or can it still be used for experiments? Is the only fix switching to Bacto Peptone like described in the earlier posts? 3.14Mb

Link to this post | posted 20 Sep, 2024 14:07

bavina

Hello,

I am also having issues with my PYCa media. I made a batch of broth and top agar and they both became cloudy after adding the CaCl2 solution. There aren't any huge flaky crystals but still a visible change the media almost immediately after adding 1M CaCl2. I waited until the solutions were cooled to 55 C but this still happened. Is this media unusable or can it still be used for experiments? Is the only fix switching to Bacto Peptone like described in the earlier posts?

Posted in: Phage Discovery/Isolation → Precipitation of CaCl2 in PYCA top-agar