Is it possible to get fresh A. globiformis from UPitt? Would I need to go through all the legal hoops again to do so? Our lab techs believe that when we received our original stock last summer that it was stuck in the freezer immediately and that is causing our problems. I am thinking of asking MCC if they will give us some host.

We have been seeing plaques on plates only to lose them when we try to purify/amplify from webbed plates. Checking the forums I saw that we have probably been working with a contaminant since Phage Liebe will not infect the bacterial lawn. Streaking out our stock yielded no growth. Streaking out what we though was Arthrobacter yields two different morphologies. One is clearly a contaminant and the other I hope is Arthrobacter, but won't know until later this week.