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All posts created by RyanWheeler
| Link to this post | posted 19 Aug, 2024 16:45 | |
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Hi, That's the exact problem I had. It came from a contaminant in the M. foliorum stocks. I streaked a new plate and selected a single colony, which I used to streak a new plate and select another single colony and grew up that colony for fresh bacteria stocks. This solved the problem for me. -Ryan |
| Link to this post | posted 16 Feb, 2024 14:01 | |
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Hi, I pulled my high titer lysates from the -80 and did a spot test and had excellent results. The spot was completely clear of the host (M. foliorum) and I took this to mean that the phage had survived the -80 storage. However, when I tried to do a titer I had no plaques on any of my plates. I tried this twice, first by picking the spot around the margins, and second by using the lysate directly. Neither produced plaques, and I used the same plates, top agar, phage buffer, and M. foliorum culture that I used in the successful spot test. I'm not sure what I'm doing wrong in the titer. Any help would be appreciated. Thanks, Ryan |
| Link to this post | posted 05 Dec, 2023 18:37 | |
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Hello everyone, I am looking purchase materials for the DNA extraction and I am a little stuck on the item "nuclease mix". Does this refer to a mix of DNase and RNase or some specific product? Which brand of either works the best in your experience? Thanks, Ryan |
Posted in: Phage Discovery/Isolation → Best nuclease mix?