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All posts created by JChahal

| posted 28 Sep, 2020 15:27
Thank you! I'll let her know smile
Jasmin
Posted in: SEA-PHAGES Virtual MachineSEA-PHAGES Virtual Machine Error message
| posted 28 Sep, 2020 04:33
Thanks!

Jasmin
Posted in: Functional Annotationcluster K terminases
| posted 28 Sep, 2020 04:31
Hey,
My student has an issue with her virtual box.

Every time she opens up her virtual box, it won’t let her scale up to the full windows screen (see attachment). She is unable to change the settings and can't log in.
She tried to set it up again and that didn't work. It won't let her sign in and she cannot access her windows. Not sure if someone has had the same issue or knows how to fix it?
Thanks!
Jasmin
Posted in: SEA-PHAGES Virtual MachineSEA-PHAGES Virtual Machine Error message
| posted 21 Sep, 2020 22:30
Hey all!

I'm working on annotating a cluster K1 phage (Blizzard). Gene 3 (476 to 730 bp forward) is a member of pham 26863, where some this particular gene was called as "AAA-ATPase" while in other cases, it was NKF. When looking at the NCBI Blastp, the first hit is "hypothetical protein," with 100% identity, e-value of 7e-53. However, the second hit was AAA-ATPase, with a 98.81% identity and e-value of 3e-52. The rest of the hits are a mix of hypothetical protein and AAA-ATPase. When looking at HHPred, the hits show terminase, or large subunit of terminase, all with a probability above 90% and e-value close to 0. When looking at phamerator of phages of the same cluster, some don't call this gene and some call AAA-ATPase and they are 90-100% identical to my gene. I'm a bit uncertain of what function to call this gene.
Thoughts?

Thanks!
Jasmin
Posted in: Functional Annotationcluster K terminases
| posted 08 Sep, 2020 19:17
Hey!

4 of my students had "Glimmer failure" pop up when trying to auto-annotate. They have the updated version of DNA Master. Some students were able to auto-annotate so I'm not sure what the issue is?

Best,
Jasmin
Posted in: DNA MasterGlimmer Failure on Auto Annotation
| posted 05 Sep, 2020 23:38
Oh never mind! It's working now smile
Posted in: DNA MasterBlast error
| posted 05 Sep, 2020 23:24
Thanks Debbie! I have the updated version but I'm still getting the error with SSL when I try to blast just one gene.
Posted in: DNA MasterBlast error
| posted 04 Sep, 2020 00:22
Hey again,

I tried to blast just one gene and I also got Error connecting with SSL
Jasmin
Posted in: DNA MasterBlast error
| posted 03 Sep, 2020 21:30
Hello,
I'm trying to auto-annotate my phage that we got (before my class has to do it) and I pre-selected a few and it seems as though it isn't working. My progress has been at 0% for the past 3 hours and I only pre-selected 5 genes. It worked before with Ellie so I'm not sure why it's not working this time.

Thanks! Jasmin
Posted in: DNA MasterBlast error