Hi all,

Many folks ask each year about getting extra phage genomes sequenced beyond the two per section we sequence as part of SEA-PHAGES support. We at Pitt cannot do extra sequencing for payment, but there are a number of commercial options out there. That said, you'll want to try to avoid transposon-based library preps when getting new phage genomes sequenced, and one option that has experience with phages and will use the preferred library prep method is NC State.

NC State has been doing phage sequencing for a number of years with over 230 of the sequences on PhagesDB having been sequenced there. The limitation they're working with, however, is getting up to a "critical mass" of phage samples in order to make this more niche type of run worthwhile. So turnaround can be slower if fewer people are sending samples.

Which is to say: if enough of you are interested in having more of your DNA sequenced, and you reach out to NC State (by contacting Kelly Sides, kafridey@ncsu.edu), you may get good quality data back in a relatively short amount of time. Kelly can answer any questions you have about their services.

Feel free to respond here if you're thinking of getting more samples sequenced to connect and get those numbers up.

And remember that we at Pitt are always happy to help with the assembly and QC post-sequencing, even when things are sequenced elsewhere!

–Dan
Edited today, 15:55