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Recent Activity

Reviving

Link to this post \| posted today, 19:41
stpage	Hi is there a standard protocol for reviving phage from -80 stocks? Well, I assume there is. What I mean is, what do y'all recommend?

Link to this post \| posted today, 19:57
debbie	Shallee, in a perfect world. you can take 1ul of the sample, make some dilutions, and plate for single plaques. If that doesn't work, there are some additional considerations. (and of course you can start with more than 1ul.) Second step is to pick a single plaque and make a new working lysate, being sure to verify (maybe by pcr) that you have the phage you are interested in. debbie Edited today, 19:59

Link to this post | posted today, 19:57

debbie

Shallee,
in a perfect world. you can take 1ul of the sample, make some dilutions, and plate for single plaques. If that doesn't work, there are some additional considerations. (and of course you can start with more than 1ul.)
Second step is to pick a single plaque and make a new working lysate, being sure to verify (maybe by pcr) that you have the phage you are interested in.
debbie

Edited today, 19:59