SEA-PHAGES | Minor Tail protein or Hydrolase?

Link to this post \| posted 25 Feb, 2025 22:38
uOttawaPHAGE	We are annotating phage Circuit and aren't sure how to call Circuit_Draft gene 31 (26021 - 27280). This Pham is called minor tail protein in several clusters including in comparator FH phage Bumble. However, in some phages it is called Hydrolase (for example Vibaki_31, cluster FL), and other FH phages have a different Pham in same position often called as a hydrolase (Klevey_34). Circuit has three minor tail proteins after the tape measure, and then ~10 genes before gene 31, so we weren't sure if this arrangement is OK, or if all the minor tail proteins need to be adjacent to each other.

Link to this post | posted 25 Feb, 2025 22:38

We are annotating phage Circuit and aren't sure how to call Circuit_Draft gene 31 (26021 - 27280). This Pham is called minor tail protein in several clusters including in comparator FH phage Bumble. However, in some phages it is called Hydrolase (for example Vibaki_31, cluster FL), and other FH phages have a different Pham in same position often called as a hydrolase (Klevey_34).

Circuit has three minor tail proteins after the tape measure, and then ~10 genes before gene 31, so we weren't sure if this arrangement is OK, or if all the minor tail proteins need to be adjacent to each other.

Link to this post \| posted 26 Feb, 2025 16:13
debbie	Hi Adam, hmmmm. This gene is in the region of the minor tail proteins. Minor tail proteins have hydrolase and esterase activity in them. So I was wondering if there what the distal tail looked like for cluster FH. I was hoping to see extra fibers on the end of the tail. First, I believe Cluster FH phages look to be myovirus, even though your particle could be interpreted as a podo. I would check more than one particle to be sure. Looking at the genome, that you see a tape measure protein and many minor tail proteins, I think you will find that this is not a podovirus. My HHPRed results of this gene clearly hit a domain in other phage tail genes. Its location makes me want to call it a tail gene, and my point above that minor tail genes can have hydrolase functions makes it easy for me to call this a minor tail protein. debbie

Link to this post | posted 26 Feb, 2025 16:13

debbie

Hi Adam,
hmmmm. This gene is in the region of the minor tail proteins. Minor tail proteins have hydrolase and esterase activity in them. So I was wondering if there what the distal tail looked like for cluster FH. I was hoping to see extra fibers on the end of the tail. First, I believe Cluster FH phages look to be myovirus, even though your particle could be interpreted as a podo. I would check more than one particle to be sure. Looking at the genome, that you see a tape measure protein and many minor tail proteins, I think you will find that this is not a podovirus.
My HHPRed results of this gene clearly hit a domain in other phage tail genes. Its location makes me want to call it a tail gene, and my point above that minor tail genes can have hydrolase functions makes it easy for me to call this a minor tail protein.

debbie

Link to this post \| posted today, 05:10
uOttawaPHAGE	hi Debbie OK, it sounds like it is OK for one minor tail protein to be significantly further downstream from the others. We recognized it had an activity found in tail proteins and hits with other tail proteins. We will call it as a minor tail protein. Well aware that the TEM misled the student into thinking this was a podo. We quickly saw that it was a myo and will likely repeat the TEM before the end of the term. thanks Adam

Link to this post | posted today, 05:10

uOttawaPHAGE

hi Debbie

OK, it sounds like it is OK for one minor tail protein to be significantly further downstream from the others. We recognized it had an activity found in tail proteins and hits with other tail proteins. We will call it as a minor tail protein.

Well aware that the TEM misled the student into thinking this was a podo. We quickly saw that it was a myo and will likely repeat the TEM before the end of the term.

thanks

Adam

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Minor Tail protein or Hydrolase?