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L3 frameshift

Link to this post \| posted 19 Dec, 2024 22:48
byrumc@cofc.edu	In some of the L3 phages the chaperone protein frameshift is annotated as a +1 frameshift and in others it is annotated as a -2 frameshift. When annotated as a -2 frameshift, the sequence is HFFFGSK. When annotated as a +1 frameshift the sequence is HFFGSK. Both seem equally feasible. Is there any mass spec evidence that one is more likely than the other? The slippery sequence is TTTTTTC…but it involves a shift from the 2nd reading frame to the 1st instead of a shift from the 2nd reading frame to the 3rd.

Link to this post | posted 19 Dec, 2024 22:48

byrumc@cofc.edu

In some of the L3 phages the chaperone protein frameshift is annotated as a +1 frameshift and in others it is annotated as a -2 frameshift. When annotated as a -2 frameshift, the sequence is HFFFGSK. When annotated as a +1 frameshift the sequence is HFFGSK. Both seem equally feasible. Is there any mass spec evidence that one is more likely than the other? The slippery sequence is TTTTTTC…but it involves a shift from the 2nd reading frame to the 1st instead of a shift from the 2nd reading frame to the 3rd.