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Covaris fragmentation
| Link to this post | posted yesterday, 19:24 | |
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We're having a go at library prep, but the core here apparently isn't sure where to start for non-mammalian DNA. Does anyone have settings for Covaris (E220) (we're sequencing on iSeq 2x150, shooting for fragments ~400 bp)? From restriction gels, I think our genomes are all around average size for globi phage (~40-50 kb). |