Review of this cluster at the 2023 Faculty meeting revealed many phages that have been annotated with a slippery sequence that DO NOT meet the sequences listed as validated in the Bioinformatics guide:
These include CGGGGCG (should be CGGGGGCG: note there are 5 Gs) and GGGAAA (the correct sequence is GGGAAAG, GGGAAAT or GGGAAAA, note there are FOUR nucleotides after the GGG, not three). It is essential to assure that when you call the slippery, it matches to the list in the phages guide, otherwise we have a big mess and it propagates miscalls. As more wet lab data is developed, the list is updated, so this should be checked each year you are annotating.
RS Pollenz