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why is my agar green?
| Link to this post | posted 04 Oct, 2022 15:21 | |
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We have a new issue! For one student only, when purifying phage using M.smeg her agar on her plate is turning a light green. No other contamination is evident and no other students are having the same issue. Is there something about a particular phage that might cause the agar to turn green?? For clarification - not the top agar containing phage and smeg, only the plate agar from original pour. All other plates and top agar are fine (typical color). Thanks! |
| Link to this post | posted 04 Oct, 2022 15:45 | |
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Hi Stephanie, If you are using 7H10 to make your agar, it contains malachite green and the agar should really be green. Once overcooked and autoclaved, it tends to be more tan than pale green. If this is the cause of the green, there is nothing wrong. If the agar is undercooked or autoclaved, it can appear greener, BUT you will also have no growth and/or contamination issues, which are a bigger problem. Does that fit what you are seeing? |
| Link to this post | posted 04 Oct, 2022 15:49 | |
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Maybe? There are lots of lysogenic plaques so it makes it a little harder to tell. We will filter and replate again today and see if the same thing happens! All other plates made in the same batch are tan/not greenish. My first guess is some kind of contamination but it is weird that it is not in top agar and only turned green after plating with phage. |
| Link to this post | posted 04 Oct, 2022 16:37 | |
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If further info is needed, pictures would be helpful. Good luck! |