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September Faculty Meeting Notes on Gordonia
| Link to this post | posted 16 Sep, 2022 21:04 | |
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Notes on using Gordonia as a host from the September 16, 2022 faculty meeting: Tips and tricks: Phage isolation success rate can vary; excessive rain may be reducing rates but generally wet weather is helpful Excessively saturated samples typically do not yield phages Drainage areas tend to be high-yield areas Small plaques: decrease top agar concentration (maybe to 0.3%) and reduce amount of cells Small plaques may also indicate phages that grow better on another strain of Gordonia Hosts currently used for host range Gordonia terrae CAG3, G. rubripertincta, G. westfalica, G. lacunae Collaboration ideas: Host range tests Increasing phage isolation yield Investigating how to improve growth of phages that produce small plaques |