SEA-PHAGES | Electron microscopy protocols

Electron microscopy protocols

Link to this post \| posted 05 May, 2022 17:19
bgibb	There used to be three protocols for setting up TEM samples, but now there is only one 8.1c (parafilm drop method) in the Discovery guide and Instructor guide. Is there a reason that the other protocols were removed? I've had good luck with the pelco tab method.

Link to this post \| posted 05 May, 2022 17:50
viknesh	bgibb There used to be three protocols for setting up TEM samples, but now there is only one 8.1c (parafilm drop method) in the Discovery guide and Instructor guide. Is there a reason that the other protocols were removed? I've had good luck with the pelco tab method. Bryan, I see all 3 protocols in the Discovery Guide - https://seaphagesphagediscoveryguide.helpdocsonline.com/8-0-toc

Link to this post \| posted 06 May, 2022 03:06
bgibb	You're correct– I was looking at the instructor guide instead of the discovery guide. Thanks! viknesh bgibb There used to be three protocols for setting up TEM samples, but now there is only one 8.1c (parafilm drop method) in the Discovery guide and Instructor guide. Is there a reason that the other protocols were removed? I've had good luck with the pelco tab method. Bryan, I see all 3 protocols in the Discovery Guide - https://seaphagesphagediscoveryguide.helpdocsonline.com/8-0-toc

Link to this post | posted 06 May, 2022 03:06

bgibb

You're correct– I was looking at the instructor guide instead of the discovery guide. Thanks!

viknesh
bgibb
There used to be three protocols for setting up TEM samples, but now there is only one 8.1c (parafilm drop method) in the Discovery guide and Instructor guide. Is there a reason that the other protocols were removed?

I've had good luck with the pelco tab method.

Bryan, I see all 3 protocols in the Discovery Guide - https://seaphagesphagediscoveryguide.helpdocsonline.com/8-0-toc

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Electron microscopy protocols