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byrumc@cofc.edu posted in L3 frameshift
byrumc@cofc.edu posted in L3 frameshift
Viknesh Sivanathan posted in did you know you can do restriction digests in the microwave?
nic.vega posted in did you know you can do restriction digests in the microwave?
nic.vega posted in did you know you can do restriction digests in the microwave?
Low isolation rate for Gordonia terrae this semester
| Link to this post | posted 11 Oct, 2021 17:27 | |
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Hi all- We've successfully made it through direct isolation and purification this semester, but has anyone else noticed a consistently low isolation rate for Gordonia phages over the last two months? We usually have a very high success rate (50-70%) on direct isolation here in Pittsburgh, but it's dropped down to 20% this semester across all 10 of our sections. Has anyone else noticed issues with low isolation rates for Gordonia? I'm curious as to what could be happening. |
| Link to this post | posted 23 Oct, 2021 16:09 | |
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We have had a rough time isolating any phages this semester. I tried moving to G rubripertincta. We got one phage out of 4-5 rounds with 14 students. We switched to G. terrae and have only gotten one. It’s really difficult to keep students motivated. |