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Viknesh Sivanathan posted in did you know you can do restriction digests in the microwave?
nic.vega posted in did you know you can do restriction digests in the microwave?
nic.vega posted in did you know you can do restriction digests in the microwave?
Viknesh Sivanathan posted in did you know you can do restriction digests in the microwave?
nic.vega posted in did you know you can do restriction digests in the microwave?
Upstream minor tail (pham 38688) really NKF
| Link to this post | posted 02 Jun, 2020 16:49 | |
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QCing Chivey, and it looks like we are perpetuating another bad minor tail protein call in the EF phages. Pham 38688, found in several different clusters, is a small protein (350 -400 bp) well upstream of the tapemeasure protein in EF (around gp22); a significant majority of the pham members assign NKF. Best HHPred hit (see attached) in all non-draft EF is from pfam (phage HK97-gp10) 'putative tail-component' (TIGRFAMs calls this same gene a model for an uncharacterized, highly divergent bacteriophage family); no good PDB or collagen or coiled-coiled domains. Based on this and the May 27 SMART conversation I'm changing Chivey's to NKF–heads-up for other EF. |
9Kb| Link to this post | posted 02 Jun, 2020 17:03 | |
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JoAnn, I agree. The top hit is to HK97 gp 10. This phage is well studied by the Hendrix lab here at Pitt and while this is a structural protein in that phage, its function is unknown. The second hit is to a minor capsid protein. So it is easy to assume that this is a structural gene, which one is unknown. debbie |