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Recent Activity
Cluster A3 Immunity Repressor?
| Link to this post | posted 24 Jan, 2020 20:17 | |
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In calling the immunity repressor of a phage JF2 (gp71), we came across a peculiar finding. It seems that the immunity repressor's open reading frame has less coding potential than another open reading frame in that area. Don't be fooled! JF2's immunity repressor shows hits in HHPred to the HTH DNA binding site (N-terminus). Read Pope, et al 2011a for info about Bxz2's broken repressor. However, our gene doesn't match that N terminus either. Many (did I say many?) phages have miscalled the open reading frame as the immunity repressor. Be careful. We will fix as soon as we can. debbie |
| Link to this post | posted 15 Jul, 2022 15:06 | |
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debbie Hi Debbie Is the immunity repressor called correctly in Norbert_70 and Lambert1_73. We annotated Caviar and it has a 552 base pair deletion in that region and therefore doesn't have an immunity repressor gene. Please advise Thank you Fernando |
| Link to this post | posted 15 Jul, 2022 15:06 | |
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debbie Hi Debbie Is the immunity repressor called correctly in Norbert_70 and Lambert1_73. We annotated Caviar and it has a 552 base pair deletion in that region and therefore doesn't have an immunity repressor gene. Please advise Thank you Fernando |
| Link to this post | posted 18 Jul, 2022 23:43 | |
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Fernando, Yes, the immunity repressor is called correctly in those genomes. And yes, that area is omitted in Caviar, so you most likely have a lytic phage. Likely a derivative a temperant one. debbie |
| Link to this post | posted 20 Aug, 2022 02:00 | |
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debbieDebbie Thank you Fernando |