SEA-PHAGES Logo

The official website of the HHMI Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science program.

Welcome to the forums at seaphages.org. Please feel free to ask any questions related to the SEA-PHAGES program. Any logged-in user may post new topics and reply to existing topics. If you'd like to see a new forum created, please contact us using our form or email us at info@seaphages.org.

Minor tail Protein on EA genomes

| posted 01 Aug, 2019 17:42
I'm in the process of annotating YuuY and I noticed that one of the genes is called minor tail protein (Pham 11397, coordinates 12806-15232 in Yuuy)in almost every EA cluster genome even though it is larger than the tape measure protein (Pham 11400, coordinates 9625-12036) and much larger than the major tail protein (Pham 11479, coordinates 8110-8604).

HHPRED shows 99% probability (36.7% coverage) that it matches to prophage endopeptidase tail but given the size of the gene I wanted to confirm that this is the right call.

Thank you,

Arturo
| posted 01 Aug, 2019 18:00
On a related note, gp22 (pham 17964) is also listed as a minor tail protein in most EA genomes but the HHPRED data matches Chitinase A (100% probability. I guess I'm having a hard time understanding how something that cleaves Chitin is related to a minor tail protein.

Arturo
| posted 05 Aug, 2019 22:30
I always think that an HHPRED of 100% probability is an indication that it is very confident that the protein you are searching with belongs to the same family of proteins it is matching. Chitin is a polymer of β-(1→4)-linkages of N-acetylglucosamine (NAG) while peptidoglycan is alternating residues of β-(1,4) linked N-acetylglucosamine and N-acetylmuramic acid.

I always try to remind my students that functional annotation down to one specific substrate is very risky. In my mind it is easy to change just one or two amino acids at exactly the right place to radically change the binding properties of an enzyme to any one specific substrate. And one should be even more careful when there are known to be many different but related substrates [like polysaccharides].

So annotation of this type needs a "sanity check". Does it make sense that a phage would have an enzyme that cleaves chitin? No, not really, so I would not annotate this as a chitinase. One the other hand, it does make sense that some ancient protein that does something with N-acetylglucosamine could easily evolve into a chitinase along one branch and evolve into some other protein that somehow interacts with peptidoglycan in another branch. I would come away pretty confident that this phage protein either binds to NAG or cleaves β-(1→4)-linkages of NAG.

Since there is no approved term for "protein either binds to NAG or cleaves β-(1→4)-linkages of NAG" I would stick with "minor tail protein" but I would also be pretty confident of that annotation given the structural similarity of chitin and peptidoglycan.
| posted 06 Aug, 2019 19:29
Hi Chris,

Thank you for your thorough response, that was really helpful! I'm still familiarizing myself with phage biology, it's not unusual for the genes that encode for minor tail proteins to be larger than the major tail genes?
| posted 07 Aug, 2019 19:10
I believe the "major" and "minor" terms relate back to very early papers where they purified phage proteins and ran them out on protein gels. So a "major" proteins would be the very intense band on the gel and would presumably be high copy number. There would also be weak or even very faint banks on the gel and these would be the "minor" proteins. The terms then had less to do with size but rather abundance.
 
Login to post a reply.