Welcome to the forums at seaphages.org. Please feel free to ask any questions related to the SEA-PHAGES program. Any logged-in user may post new topics and reply to existing topics. If you'd like to see a new forum created, please contact us using our form or email us at info@seaphages.org.
Recent Activity
Viknesh Sivanathan posted in did you know you can do restriction digests in the microwave?
nic.vega posted in did you know you can do restriction digests in the microwave?
nic.vega posted in did you know you can do restriction digests in the microwave?
Viknesh Sivanathan posted in did you know you can do restriction digests in the microwave?
nic.vega posted in did you know you can do restriction digests in the microwave?
G. rubri no longer turning pink in liquid or plates
| Link to this post | posted 17 Sep, 2018 15:32 | |
|---|---|
|
This year we've switched to Gordonia rubripertincta. Initial streak plates from the stock Debbie sent to us grew pink as expected, as did the subsequent P1FF and P2FF cultures. Plaque assay plates from direct isolation experiments also turned pink. This past week, however, they have failed to turn pink, and a new P1FF culture taken from the same pink P0FF streak plate as earlier grew as a tan/yellow solution after two days of growth in a shaker incubator. Incubations (including shaking) is always at 30C. I know the pink color accumulates over time, but I am trying to figure out why they are not turning pink now, when they did before. Aside from the usual things like testing new PYCa media, etc, I was wondering if anyone else has encountered a similar problem and can tell me a) should I be concerned, and b) ideas for what the problem might be, if any. |
| Link to this post | posted 17 Sep, 2018 15:55 | |
|---|---|
|
Jason, The simplest explanation is one of the following: a. you have a non-pigmented mutant b. it is a contaminant (and no longer G. rubi) I would recommend going back to the frozen stock. debbie |