Welcome to the forums at seaphages.org. Please feel free to ask any questions related to the SEA-PHAGES program. Any logged-in user may post new topics and reply to existing topics. If you'd like to see a new forum created, please contact us using our form or email us at info@seaphages.org.

Recent Activity

Reverse gene in cluster AN

Link to this post \| posted 06 Mar, 2018 23:39
kklyczek	These genomes have one reverse gene, located near the right end - see Maggie_21. Autoannotation usually misses these and instead calls a small forward gene in that location. The forward gene should be deleted, and instead insert the reverse gene, which will have blast hits to HTH DNA binding domain proteins. Autoannotation sometimes calls a different reverse gene, entirely overlapping a forward gene, near the right end. That reverse gene (seen in some current drafts, e.g. Copper_Draft_24) should be deleted. Edited 08 Mar, 2018 14:15

Link to this post | posted 06 Mar, 2018 23:39

kklyczek

These genomes have one reverse gene, located near the right end - see Maggie_21. Autoannotation usually misses these and instead calls a small forward gene in that location. The forward gene should be deleted, and instead insert the reverse gene, which will have blast hits to HTH DNA binding domain proteins.

Autoannotation sometimes calls a different reverse gene, entirely overlapping a forward gene, near the right end. That reverse gene (seen in some current drafts, e.g. Copper_Draft_24) should be deleted.

Edited 08 Mar, 2018 14:15