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lmeadows@rcbc.edu posted in 260/280 ratios too high from DNA extraction
Gene 1
| Link to this post | posted 01 Mar, 2018 15:30 | |
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Gene 1 in Cluster A phages is usually a HNH endonuclease oriented in the forwards direction. Cluster A4 phages in particular sometimes fool the gene prediction programs and put a small gene in the reverse orientation as gene 1. Don't be fooled! The reverse gene is a false prediction and should be deleted. You will likely have to manually add the real one. |