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Viknesh Sivanathan posted in did you know you can do restriction digests in the microwave?
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nic.vega posted in did you know you can do restriction digests in the microwave?
Viknesh Sivanathan posted in did you know you can do restriction digests in the microwave?
nic.vega posted in did you know you can do restriction digests in the microwave?
DNA isolation w/Zn precipitation
| Link to this post | posted 06 Jul, 2017 20:57 | |
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Hi everyone: At the symposium last month someone spoke about a column-less method of DNA extraction using a Zn precipitation. I looked up the paper and it seems we don' have access to the whole thing without a lot of gyrations. Can anyone send me the protocol? It would be much appreciated. |
| Link to this post | posted 12 Oct, 2018 14:34 | |
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This is the protocol we use, based upon that publication. It worked well for us last year with M. smeg, and we'll be trying it this year with M. foliorum. The EDTA step we just added, in hopes that it will help with some of the degradation issues the foliorum people seem to have had. Sorry it's coming so late, but perhaps it'll still be useful! |
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