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Viknesh Sivanathan posted in did you know you can do restriction digests in the microwave?
nic.vega posted in did you know you can do restriction digests in the microwave?
nic.vega posted in did you know you can do restriction digests in the microwave?
Viknesh Sivanathan posted in did you know you can do restriction digests in the microwave?
nic.vega posted in did you know you can do restriction digests in the microwave?
Patch test experiment
| Link to this post | posted 10 Apr, 2017 22:08 | |
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Some of our students are investigating potential lysogens. We have 2 questions: 1. Do we need to use 7H10 plates for the patch assay lysogen only plate, or can we use L-Agar plates without top agar? (if we do need the 7H10 plates, why is that?) 2. Can use use a wire loop rather than disposable loops? Thanks, Susan |
| Link to this post | posted 11 Apr, 2017 15:14 | |
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Susan, You can do the patch test on L-agar. Sure, why not! I don't understand the without top agar. the patch test is done to visualize phage release from a colony. So you need wild type smeg in the top agar for that detection. You make a companion plate with the patch test, so that your can carry that colony forward (without picking up WT smeg. does that make sense? You can always use a wire loop. Depending on numbers, that is a lot of firing the loop. You can also use sterile toothpicks for some of this testing. |
| Link to this post | posted 11 Apr, 2017 23:32 | |
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Thank you Debbie, that makes sense! Susan |