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Viknesh Sivanathan posted in did you know you can do restriction digests in the microwave?
nic.vega posted in did you know you can do restriction digests in the microwave?
nic.vega posted in did you know you can do restriction digests in the microwave?
Viknesh Sivanathan posted in did you know you can do restriction digests in the microwave?
nic.vega posted in did you know you can do restriction digests in the microwave?
shelf life of "fluid" 7H9
| Link to this post | posted 05 Aug, 2016 15:29 | |
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As I guess many/most of us do, we make top agar in two components: a 2XL agar and what we refer to as a "fluid" portion which is just 7H9. We have a fair bit left over from last fall that was probably made back in late October. Does anyone have a handle on the shelf life of this stuff at room temperature? I'd hate to throw it out if it's still perfectly good, but it concerns me because I think it contains a fair number of low molecular weight organic compounds that may not do well in solution over time. |
| Link to this post | posted 06 Aug, 2016 12:11 | |
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Joe, HI! If you haven't lost volume (water), I think you are OK. If you have dropped the volume significantly, you might want to add some sterile water. I don't think you will have trouble with the degradation of small molecules. Just include its date in your lab notebook, so if a pattern presents itself, you will be able to link it to this component. |