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Viknesh Sivanathan posted in did you know you can do restriction digests in the microwave?
nic.vega posted in did you know you can do restriction digests in the microwave?
nic.vega posted in did you know you can do restriction digests in the microwave?
Viknesh Sivanathan posted in did you know you can do restriction digests in the microwave?
nic.vega posted in did you know you can do restriction digests in the microwave?
Tail assembly chaperones?
| Link to this post | posted 08 Feb, 2023 01:52 | |
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Does anyone have any thoughts about this potential TAC gene in B1 phages? I'm going to submit my annotation soon and would love to finalize this gene before they get to SMART. Please see my attachment in the above post for all the info. Best, Amanda |
| Link to this post | posted 08 Feb, 2023 03:15 | |
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Amanda, I vote for not calling any TACs. debbie |
| Link to this post | posted 08 Feb, 2023 16:28 | |
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Thank you Debbie! |
| Link to this post | posted 26 Jun, 2024 16:23 | |
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My class annotated the cluster B1 phage Schueller. We too saw the odd genome organization immediately upstream of the tape measure protein: forward, forward, reverse. Analysis of both forward genes match what was seen in Usavi (see previous posts). Additionally, I used alphafold 3 and ChimeraX to fold and display one of the reference TACs (3D8I.pdb) and schueller gp_25 (2nd forward gene with partial hits to TACs), and then to merge the structures. Photos are attached showing little structural similarity and virtually no overlap when the predicted structures are merged; Blue is the pdb reference tan is schueller. Conclusion: these genes with partial hits to TACs are not TACs, and no TACs should be called. |
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