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Recent Activity
tail assembly chaperones
| Link to this post | posted 24 May, 2022 15:28 | |
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Hi all. Seems that the TAC ribosomal slippage is difficult to locate. Do not call the slippage at CCCGGAA. It is not a canonical choice. However, recognize that there are some members in Subcluster DN3 and DN4 (Apricot, for example) that do have a canonical slippage, namely G_GG.G_GA.A (where _ separates codons in the original frame, and . separates them in the new frame). |
| Link to this post | posted 07 Jun, 2023 14:56 | |
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A follow up on Debbie's post on TACs in cluster DNs written by SEA faculty at the 2023 Faculty member: Not all cluster DN phage genomes have two tail assembly chaperones (TAC) that can be called and a programmed ribosomal frameshift (PRF) that can be called. An example of a DN phage with a non-canonical -1 programmed translational frameshift (CCCGGAA) is Horus gp 15 and gp 16 and the PRF should not be called. If no PRF can be called and there is no HHpred evidence supporting a TAC function assignment then only one TAC chaperone should be called. An example of a DN phage with a canonical -1 programmed translational frameshift (GGGGGAA) is found in Oday TAC gp14. Although there is no HHpred evidence for a TAC in Oday gp15, because the canonical slippery site is present in gp14, gp15 is called a TAC. |