SEA-PHAGES Logo

The official website of the HHMI Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science program.

Welcome to the forums at seaphages.org. Please feel free to ask any questions related to the SEA-PHAGES program. Any logged-in user may post new topics and reply to existing topics. If you'd like to see a new forum created, please contact us using our form or email us at info@seaphages.org.

Electron microscopy protocols

| posted 05 May, 2022 17:19
There used to be three protocols for setting up TEM samples, but now there is only one 8.1c (parafilm drop method) in the Discovery guide and Instructor guide. Is there a reason that the other protocols were removed?

I've had good luck with the pelco tab method.
| posted 05 May, 2022 17:50
bgibb
There used to be three protocols for setting up TEM samples, but now there is only one 8.1c (parafilm drop method) in the Discovery guide and Instructor guide. Is there a reason that the other protocols were removed?

I've had good luck with the pelco tab method.

Bryan, I see all 3 protocols in the Discovery Guide - https://seaphagesphagediscoveryguide.helpdocsonline.com/8-0-toc
| posted 06 May, 2022 03:06
You're correct– I was looking at the instructor guide instead of the discovery guide. Thanks!
viknesh
bgibb
There used to be three protocols for setting up TEM samples, but now there is only one 8.1c (parafilm drop method) in the Discovery guide and Instructor guide. Is there a reason that the other protocols were removed?

I've had good luck with the pelco tab method.

Bryan, I see all 3 protocols in the Discovery Guide - https://seaphagesphagediscoveryguide.helpdocsonline.com/8-0-toc
 
Login to post a reply.