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Recent Activity
Patch Assay Help
| Link to this post | posted 15 Feb, 2022 16:15 | |
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Good morning. I have question concerning the patch assay. Each time I have performed the patch assay, there is growth on where the potential lysogen should be. Instead of having clearing on where my potential lysogen should be, I keep having growth. I am not sure what to do next. I have been following the protocol stated on 11.2 of the phage discovery guide. |
1.94Mb| Link to this post | posted 15 Feb, 2022 16:27 | |
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Hi! You should have bacterial growth! But if it is a lysogen, that bacterial growth should be releasing phage, so around the bacteria streak you will see a zone of lysis. You can see a tiny zone of lysis around A and D. But it is not easy to tell. What phage are you working with? debbie |
| Link to this post | posted 15 Feb, 2022 17:58 | |
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The bacteria that I am using in the research is Arthobacter golbiformis, and the lysate is called Basilisk. |
| Link to this post | posted 15 Feb, 2022 18:18 | |
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Next step, is to test the grow a culture from a streak on WT A. globi. (I would pick D.) From that culture, you will want to make a lawn and then test the phage for infection (by plating a serial 10-fold dilution) AND spinning some of the cells down and testing (by plating a serial dilution) the supernatant on the WT A. globi. Make senee? debbie |
| Link to this post | posted 15 Feb, 2022 18:32 | |
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I would just do the protocol of 11.3, right? |
| Link to this post | posted 15 Feb, 2022 20:11 | |
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yep! |