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Viknesh Sivanathan posted in did you know you can do restriction digests in the microwave?
nic.vega posted in did you know you can do restriction digests in the microwave?
nic.vega posted in did you know you can do restriction digests in the microwave?
Viknesh Sivanathan posted in did you know you can do restriction digests in the microwave?
nic.vega posted in did you know you can do restriction digests in the microwave?
TTG followed by ATG
| Link to this post | posted 03 Jul, 2018 19:53 | |
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In Sombrero, I have found a couple of genes where there is a TTG start followed by ATG. Glimmer and GeneMark both pick the ATG as the start. The same genes in other CS2 phage have mostly been annotated with the TTG start. The RBS Z-score is the same for both but the final score goes down a little for the TTG start. Given that TTG is rarely used, is it correct to assign TTG as the start in this situation? |
| Link to this post | posted 05 Jul, 2018 20:11 | |
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Hi Chris, All of the mass-spec evidence that we have indicates that when there are two start codons back-to-back, it is the downstream one that is correct. So sounds like you should go with the ATG in this case. Best, Welkin |
| Link to this post | posted 16 Apr, 2020 18:25 | |
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welkin Does this apply to all codons (any combination of ATG/TTG/GTG back to back)? Thanks! |
| Link to this post | posted 26 Feb, 2023 19:16 | |
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Yes. See Feb. 23 revision to the Guiding principles point 12.f. |