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uOttawaPHAGE posted in frameshifts
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ACMPhageHunters posted in Clarification Question About HNH Endonuclease Function Determination in view of hits to the Ref Sequences
cdshaffer posted in Clarification Question About HNH Endonuclease Function Determination in view of hits to the Ref Sequences
frameshifts
| Link to this post | posted 14 Mar, 2018 14:41 | |
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Cluster F phages can have either +1 or -1 frameshifts in the tail assembly chaperones. Make sure you know which one your phage has. |
| Link to this post | posted today, 03:08 | |
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We are annotating Lordvader (Cluster F) and the sequence and slippery sequence of the tail assembly chaperone matches Frankie (F1): KS to KV (LordVader: 8391..8885,8887..9327; Frankie: 8588..9082,9084..9524), with a slippery sequence of CCAAAAG. This sequence is found on the table in the guide as being used in IS3 as a -1 slip, but this is a +1 slip. I haven't seen any discussion on the forums of evidence to support the +1 slip using this sequence. It seems like others have called it, but I wanted to confirm. |