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what on earth is this thing (TEM)

| posted yesterday, 23:52
TEM image attached of one of our problem children this semester. In this view, it looks like there are a bunch of destroyed capsids (which tracks, this phage has been a challenge to keep alive) and possibly one intact, very round capsid against a field of ribosomes… but if so, the capsid is TINY. Is this even a phage?

Host is Arthrobacter globiformis B-2979.
| posted today, 02:06
Nic,
Check out this EM.
or phage Piku.
Not sure what is in the picture is the phage, but I think I see similar structures in these micrographs.

debbie
| posted today, 15:33
Hi Nic: the dark things in your micrograph are ribosomes.I suspect the bright shiny sphere is a vesicle, perhaps.
Carol
| posted today, 16:22
Those do look very similar! Not a phage, then… microvesicle or lipid droplet makes sense. Which means we did somehow blow up all the capsids, this was an e10 stock!
 
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