SEA-PHAGES

Link to this post \| posted 07 Nov, 2025 14:42
liltye01	Good morning All, Would someone be able to point me in the right direction to find the TEM information from HHMI. I remember when we were in Maryland we discussed an option to send our samples back there for TEM and we are at that point now, so I need the contact information, mailing address and procedures for packing. Thanks

Link to this post \| posted 07 Nov, 2025 14:48
viknesh	liltye01 Good morning All, Would someone be able to point me in the right direction to find the TEM information from HHMI. I remember when we were in Maryland we discussed an option to send our samples back there for TEM and we are at that point now, so I need the contact information, mailing address and procedures for packing. Thanks You are referring to the Imaging Facilty run by UMBC, which images phages for many schools running SEA-PHAGES but it is otherwise not affiliated with HHMI. Their phage imaging services (pricing etc.) can be found on their website, here: https://kpif.umbc.edu/bacteriophage-imaging/

Link to this post | posted 07 Nov, 2025 14:48

viknesh

liltye01
Good morning All,
Would someone be able to point me in the right direction to find the TEM information from HHMI. I remember when we were in Maryland we discussed an option to send our samples back there for TEM and we are at that point now, so I need the contact information, mailing address and procedures for packing.

Thanks

You are referring to the Imaging Facilty run by UMBC, which images phages for many schools running SEA-PHAGES but it is otherwise not affiliated with HHMI. Their phage imaging services (pricing etc.) can be found on their website, here: https://kpif.umbc.edu/bacteriophage-imaging/

Link to this post \| posted 10 Nov, 2025 20:05
liltye01	Thanks so much, that was very helpful.

Link to this post \| posted today, 15:18
alexander.baumann@tccd.edu	Hello, After we prepare our grids, is there a suggested time in which to image them, and a maximum time we can store them before they're, on average, too degraded for TEM to visualize the bacteriophages?

Link to this post \| posted today, 15:21
viknesh	alexander.baumann@tccd.edu Hello, After we prepare our grids, is there a suggested time in which to image them, and a maximum time we can store them before they're, on average, too degraded for TEM to visualize the bacteriophages? The grids should be stable for weeks to months, if not years. Once stained, you'll want to avoid exposure to moiture and direct light. You could put the grids (in their holder) in a small box with a dessicant.

Link to this post | posted today, 15:21

viknesh

alexander.baumann@tccd.edu
Hello,

After we prepare our grids, is there a suggested time in which to image them, and a maximum time we can store them before they're, on average, too degraded for TEM to visualize the bacteriophages?

The grids should be stable for weeks to months, if not years. Once stained, you'll want to avoid exposure to moiture and direct light. You could put the grids (in their holder) in a small box with a dessicant.

Link to this post \| posted today, 15:23
alexander.baumann@tccd.edu	viknesh alexander.baumann@tccd.edu Hello, After we prepare our grids, is there a suggested time in which to image them, and a maximum time we can store them before they're, on average, too degraded for TEM to visualize the bacteriophages? The grids should be stable for weeks to months, if not years. Once stained, you'll want to avoid exposure to moiture and direct light. You could put the grids (in their holder) in a small box with a dessicant. Thank you!

Link to this post | posted today, 15:23

alexander.baumann@tccd.edu

viknesh
alexander.baumann@tccd.edu
Hello,

After we prepare our grids, is there a suggested time in which to image them, and a maximum time we can store them before they're, on average, too degraded for TEM to visualize the bacteriophages?

The grids should be stable for weeks to months, if not years. Once stained, you'll want to avoid exposure to moiture and direct light. You could put the grids (in their holder) in a small box with a dessicant.

Thank you!

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