jayadasgupta
Hello everyone,
I have been getting broth cultures ready from agar plates (isolated colonies). I was wondering if it is ok to subculture the broth Arthrobacter for next day's experiments? Meaning, take 1ml of the overnight broth and add to 50ml fresh broth for next day's lab? Or is that a no-no? And that we should start from the plate every time?.

Thank you,
Jaya.

viknesh

jayadasgupta
Hello everyone,
I have been getting broth cultures ready from agar plates (isolated colonies). I was wondering if it is ok to subculture the broth Arthrobacter for next day's experiments? Meaning, take 1ml of the overnight broth and add to 50ml fresh broth for next day's lab? Or is that a no-no? And that we should start from the plate every time?.

Thank you,
Jaya.

The recommendation is to minimize the number of passages, as each passage increases the genetic variation in the population and possibly phenotypes important for infection. For a reliable culture, we recommend always starting a culture from a colonies off a freshly streaked plate. In a pinch, you can subculture from an existing culture, but I would do this regularly.

viknesh

jayadasgupta
Hello everyone,
I have been getting broth cultures ready from agar plates (isolated colonies). I was wondering if it is ok to subculture the broth Arthrobacter for next day's experiments? Meaning, take 1ml of the overnight broth and add to 50ml fresh broth for next day's lab? Or is that a no-no? And that we should start from the plate every time?.

Thank you,
Jaya.

The recommendation is to minimize the number of passages, as each passage increases the genetic variation in the population and possibly phenotypes important for infection. For a reliable culture, we recommend always starting a culture from a colonies off a freshly streaked plate. In a pinch, you can subculture from an existing culture, but I would do this regularly.

viknesh

jayadasgupta
Hello everyone,
I have been getting broth cultures ready from agar plates (isolated colonies). I was wondering if it is ok to subculture the broth Arthrobacter for next day's experiments? Meaning, take 1ml of the overnight broth and add to 50ml fresh broth for next day's lab? Or is that a no-no? And that we should start from the plate every time?.

Thank you,
Jaya.

The recommendation is to minimize the number of passages, as each passage increases the genetic variation in the population and possibly phenotypes important for infection. For a reliable culture, we recommend always starting a culture from a colonies off a freshly streaked plate. In a pinch, you can subculture from an existing culture, but I would do this regularly.

viknesh

jayadasgupta
Hello everyone,
I have been getting broth cultures ready from agar plates (isolated colonies). I was wondering if it is ok to subculture the broth Arthrobacter for next day's experiments? Meaning, take 1ml of the overnight broth and add to 50ml fresh broth for next day's lab? Or is that a no-no? And that we should start from the plate every time?.

Thank you,
Jaya.

The recommendation is to minimize the number of passages, as each passage increases the genetic variation in the population and possibly phenotypes important for infection. For a reliable culture, we recommend always starting a culture from a colonies off a freshly streaked plate. In a pinch, you can subculture from an existing culture, but I would do this regularly.

viknesh

jayadasgupta
Hello everyone,
I have been getting broth cultures ready from agar plates (isolated colonies). I was wondering if it is ok to subculture the broth Arthrobacter for next day's experiments? Meaning, take 1ml of the overnight broth and add to 50ml fresh broth for next day's lab? Or is that a no-no? And that we should start from the plate every time?.

Thank you,
Jaya.

The recommendation is to minimize the number of passages, as each passage increases the genetic variation in the population and possibly phenotypes important for infection. For a reliable culture, we recommend always starting a culture from a colonies off a freshly streaked plate. In a pinch, you can subculture from an existing culture, but I would do this regularly.

viknesh

jayadasgupta
Hello everyone,
I have been getting broth cultures ready from agar plates (isolated colonies). I was wondering if it is ok to subculture the broth Arthrobacter for next day's experiments? Meaning, take 1ml of the overnight broth and add to 50ml fresh broth for next day's lab? Or is that a no-no? And that we should start from the plate every time?.

Thank you,
Jaya.

The recommendation is to minimize the number of passages, as each passage increases the genetic variation in the population and possibly phenotypes important for infection. For a reliable culture, we recommend always starting a culture from a colonies off a freshly streaked plate. In a pinch, you can subculture from an existing culture, but I would do this regularly.

viknesh

jayadasgupta
Hello everyone,
I have been getting broth cultures ready from agar plates (isolated colonies). I was wondering if it is ok to subculture the broth Arthrobacter for next day's experiments? Meaning, take 1ml of the overnight broth and add to 50ml fresh broth for next day's lab? Or is that a no-no? And that we should start from the plate every time?.

Thank you,
Jaya.

The recommendation is to minimize the number of passages, as each passage increases the genetic variation in the population and possibly phenotypes important for infection. For a reliable culture, we recommend always starting a culture from a colonies off a freshly streaked plate. In a pinch, you can subculture from an existing culture, but I would do this regularly.