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Recent Activity
frameshift
| Link to this post | posted 21 May, 2018 20:13 | |
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We think we have a translational frameshift of the tail assembly protein. We found the slippery sequence GGGAAA and annotated the two regions. When validating, we get a message saying the two genes share a 5' coordinate. Are we supposed to delete the first gene after editing the second gene to have 2 regions? this gets rid of the error, but I don't see that in the instructions. |
| Link to this post | posted 22 May, 2018 00:07 | |
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That message is a warning not an error. It comes up because it is easy to get duplicate genes if you use the DNA Master tool to merge multiple DNA Master files. In this case however, you have two genes, a short form and a long form of the TAC with the same 5' end and this is exactly what you want. So it is OK to ignore the warning. |
| Link to this post | posted 22 May, 2018 01:10 | |
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Awesome. Thanks chris |
| Link to this post | posted yesterday, 15:25 | |
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This is Kissaou posting this message regarding bacteriophage: "AzulaCat" · Cluster G · 41901 bp. I am working on the frameshift and realized that I have two slippery sequences: GGGGGAAG (from 11002 - 1100  and GGGAAAAT (from 11027 - 11033). My question nos is which one to use to fix the frameshift? Any advice?Thanks Kissaou |