SEA-PHAGES Logo

The official website of the HHMI Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science program.

Abstract Summary

Below is a summary of the abstract you submitted. Presenting author(s) is shown in bold.

If any changes need to be made, you can modify the abstract or change the authors.

You can also download a .docx version of this abstract.

If there are any problems, please email Dan at dar78@pitt.edu and he'll take care of them!

This abstract was last modified on March 15, 2022 at 9:32 p.m..

University of Detroit Mercy
Corresponding Faculty Member: Stephanie Conant, conantsb@udmercy.edu
This abstract will NOT be considered for a talk.
Isolation and characterization of the B1 bacteriophages Etaye and LostandPhound
Rayan Akhdar, Jannath Aurfan, Kennedy Dunlap, Fatima Herrera, Ahmad Issa, Meghan Johnson, Bilal Kachir, Nadia Khan, Gabby Makonnen, Cyndi Park, Jungwhan Park, Lily Park, Jude Rodriguez, Alexis Sleison, Xavier Sterling, Stephanie B Conant, Jonathan S Finkel, Jacob D Kagey

The University of Detroit Mercy SEA PHAGES cohort isolated two lytic B1 bacteriophages from Mycobaterium smegmatis, Etaye and Lostandphound. Both Phages were isolated from soil samples in Michigan in the fall of 2021. Characterization of the Phages identified both as Siphoviridae phage with a lytic lifecycle. DNA was isolated from both phage and they were sequenced in preparation for genomic annotation. Etaye has a genome size of 68,950 bp and is most closely related to the annotated B1 Phage Weher20. LostandPhound has a genome size of 68,865 bp and is most closely related to the annotated B1 Phage Chorkpop. Currently, we are annotated both genomes in teams utilizing DNA Master, Phamerator, BLAST, Starterator, and Hhpred. During this semester we will finalize the annotation of these phage genomes for submission to Genbank and Phagesdb.