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This abstract was last modified on March 15, 2022 at 6:35 a.m..

Columbia State Community College
Corresponding Faculty Member: Elvira Eivazova, eeivazova@columbiastate.edu
This abstract will NOT be considered for a talk.
Genome Analysis of the AN Cluster Bacteriophage Kels
Owen Rochenbach, Adrianna Sasser, Miriam Galindo, Annaleisa Matzirakis, Alissa Williams, Wilson Myers, Braedyn Hollingsworth, Autumn Taylor, Cameron Bolton, Leonard Staggs, Elvira Eivazova

The goal of this study was to annotate the novel bacteriophage Kels belonging to the AN cluster of Actinobacteriophages. The AN cluster includes 39 phage members, with the average genome size of 15,532bp. Phages in this cluster appear to be lacking the tRNA genes. The genome of phage Kels, discovered in Pittsburgh, PA, is 14830 bp long, has the GC content of 65.4%, and composed of 23 genes. This bacteriophage, isolated on the Arthrobacter globiformis host, utilizes the lytic cycle. Phage Kels was compared to other members of the corresponding cluster to evaluate the gene content similarity. Comparative genome analysis of all AN cluster phages demonstrated a relatively low variation in GCS, ranging between 69.1% and 65.3%. The highest level of GCS of 65.3% was noted between phage Kels and phage Toulouse, discovered and characterized in Hudson, WI earlier. Genome analysis and annotation were performed using PECAAN, Phamerator, Starterator, as well as run through the DNAmaster annotation software tool. We conclude that in spite of the differences in geographical distribution, AN cluster phage genomes exhibit significant genome and gene function similarities.