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This abstract was last modified on March 16, 2021 at 11:07 a.m..

Virginia Tech
Corresponding Faculty Member: Stephanie Voshell
This abstract will NOT be considered for a talk.
Mycobacteriophage Duplicity: A temperate N cluster phage
Kirin Anand, Arya Malek, Suzette L Beeman, Sarah E Bekhet, Romina Boortalary, Rees T Bowen, Alexis K Correll, Harpreet Dhami, Samantha D Edwards, Joshua Frahm, Lexie A Goad, Matthew G Heldmann, Fadi N Hindi, James B Jorgensen, Tom R Kasputis, Allison M Lenert, Jeremy J Luers, Mackenzie A Marrella, Kiley J Martin, Morgane E McDonald, Kaitlyn E Moise, Olivia C O'Connor, Graham D Poynter, Jayali R Samarasinghe, Troy M Scott, Rania E Smeltz, Jing G Waid, Dagen K Young, Stephanie M Voshell

Mycobacteriophage Duplicity was annotated by Phage Hunters students at Virginia Tech during the Spring 2021 semester. Duplicity was originally isolated in 2014 by Phage Hunters students at Ouachita Baptist University (Arkadelphia, AR) from local soil using Mycobacterium smegmatis as a host. Duplicity is an N cluster phage with a mosaic genome. Duplicity shares similarities with N cluster phages Aggie, Charlie, and Tapioca in the left-hand side of the genome and Schnauzer and Smurph in the right-hand side of the genome. The presence of a tyrosine integrase gene suggests Duplicity is a temperate phage. Duplicity, Smurph, and Schnauzer all lack a large DNA methylase gene found in other N cluster phages.