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This abstract was last modified on June 7, 2018 at 11:11 a.m..

Fayetteville State University
Corresponding Faculty Member: Kristen Delaney-Nguyen, kdelaney@uncfsu.edu
This abstract will NOT be considered for a talk.
Isolation and Characterization of Four Novel Mycobacterium Phages from the Sandhills Region of North Carolina
Lorette Rivera-Lugo, Wendy Adams, Brittany Anderson, Korina Baldazotes, Tameka Colden, Chaselyn Ladd, Christopher Livingston, Jamahl Martin, Troi Miller, Kelsey Mohr, Gorpu Ngumah, Naimah Page, Daniya Pate, Loletha Porter, Tilar Stanford, Sangita Subedi, Jza-Qera Thompson, Nickolas Torres, Cynthia Waller, Ariana Wilson, Kristen Delaney Nguyen

Bacteriophages are the most numerous microbes in the biome and display massive genetic diversity. Thirteen phages capable of replication in Mycobacterium smegmatis mc<sup>2</sup>155 were isolated in the Sandhills region of North Carolina. Four phages (Datway, FrayBell, Leogania, and Lephleur) were sequenced at the Pittsburgh Bacteriophage Institute. All four of the sequenced phages were novel. Fraybell demonstrated myoviridae morphology and Leogania was siphoviridae. Datway and Leogania appear to be temperate phages due to their turbid plaques and the presence of lysogeny-associated genes such as integrase and immunity repressor. Continuing work will involve seeking related phages from the same geographic region using Datway and Leogania lysogens.