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This abstract was last modified on March 31, 2025 at 4:49 p.m..
Early College students, who are in their junior year in high school but are also freshmen in college, isolated, purified, and characterized multiple bacteriophages from soil samples from their backyards and the Montgomery College campus in Rockville, Maryland. They did enriched isolation using the host Arthrobacter globiformis, followed by purification, amplification, and titer determination of phage lysates. They isolated DNA and performed a restriction analysis of phage genomes. Finally, they obtained transmission electron microscope images of their phages. This research will be continued with genome annotation of select phages in the future. This work will contribute to the knowledge of phage diversity and may have potential applications in biotechnology, including in the treatment of antibiotic-resistant infections.