Below is a summary of the abstract you submitted. Presenting author(s) is shown in bold.
If any changes need to be made, you can modify the abstract or change the authors.
You can also download a .docx version of this abstract.
If there are any problems, please email Dan at dar78@pitt.edu and he'll take care of them!
This abstract was last modified on March 18, 2025 at 7:12 p.m..
Bacteriophages are viruses that infect bacteria, with potential applications in agriculture, food safety, and phage therapy. This study presents the discovery of a novel bacteriophage, AmiCi24, extracted from soil in Sewell, NJ, using the bacterium Arthrobacter globiformis. AmiCi24 was isolated through enriched isolation of a soil sample, purified through two rounds of serial dilutions and plaque assays, and amplified to collect phage lysate. Transmission electron microscope images revealed that AmiCi24 is a Siphoviridae phage with a long 60 nm tail and a 120 nm icosahedral head. The AmiCi24 genome is composed of double-stranded DNA. It is 38,466 bp in length with 66.1% GC content and 68 putative proteins, including two terminase proteins, a major tail protein, and a portal protein. Based on this analysis, AmiCi24 was classified as a cluster AS3 temperate phage. In conclusion, this research successfully characterized the newly discovered bacteriophage AmiCi24, contributing to the growing body of knowledge on bacteriophages.