SEA-PHAGES Logo

The official website of the HHMI Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science program.

Abstract Summary

Below is a summary of the abstract you submitted. Presenting author(s) is shown in bold.

If any changes need to be made, you can modify the abstract or change the authors.

You can also download a .docx version of this abstract.

If there are any problems, please email Dan at dar78@pitt.edu and he'll take care of them!

This abstract was last modified on March 30, 2023 at 10:11 p.m..

University of Ottawa
Corresponding Faculty Member: Adam Rudner, arudner@uottawa.ca
This abstract will NOT be considered for a talk.
Host Range of Celery, a BD3 Bacteriophage that Infects Streptomyces coelicolor.
Abdulla Al Aidie, Sarah Alsayadi, Kieran Chalmers, Lisa Chen, Hiba A Chentoufi, Nazia Hassan, Siyona Kassel, Katia Koziel Ly, Mariam Mahran, Zachary Mitchell, Paul Nguyen, Ayesha Syed, Fatima Sheikh-Mohamoud, Xeius Tran-Wong, Elijah Van Dinther, Keith Wheaton, Kin Chan, Elizabeth Williams, Erika Znamenski, Adam D Rudner

A cluster BD3 Siphoviridae phage, Celery, was discovered at the Faculty of Medicine campus of the University of Ottawa in Fall of 2022 and isolated using the host Streptomyces coelicolor M145. Illumina sequencing revealed a genome of 48562 bp with 65.4% GC content. The genome was annotated using PECAAN, Phamerator, Glimmer, GeneMark, Starterator, HHPred, NCBI BLAST, DeepTMHMM and PhagesDB. Automated annotation detects 79 genes with 2 orphams. NCBI BLASTN revealed high synteny with BD3 phages Amela and Verse, both with 95% query cover. These phages were found far from Ottawa at the University of North Texas, in Dallas. Amela and Verse, however, were isolated using a different host, Streptomyces venezuelae ATCC 10712, suggesting that Celery may be able to infect this host. Preliminary tests, however, suggest that Celery has a limited host range and does not infect S. venezuelae.